In vitro establishment from mature nodal segments of Persian Lime (Citrus latifolia Tan.)

Humberto Estrella-Maldonado 1, *, Julio Ventura-Bello 2, Ricardo Santillán-Mendoza 1, Cristian Matilde-Hernández 1, Arianna Chan-León 3 and Felipe Roberto Flores-de la Rosa 1

1 National Institute for Research on Agriculture, Forestry and Livestock (INIFAP). Ixtacuaco Experimental Field, Km 4.5. Martínez de la Torre-Tlapacoyan Street, Cong. Rojo Gómez, Tlapacoyan, Veracruz, Mexico.
2 University for Wellbeing “Benito Juárez García. Street University Loc. 1, Aguardientera Section Second, C.P. 73956, Chignautla, Puebla, Mexico.
3 National Technological Institute of Mexico, Campus Úrsulo Galván, Km 4.5 Street City Cardel-Chachalacas, Úrsulo Galván C.P. 91667, Veracruz, Mexico.
 
Research Article
International Journal of Science and Research Archive, 2024, 12(01), 854–861.
Article DOI: 10.30574/ijsra.2024.12.1.0868
Publication history: 
Received on 02 April 2024; revised on 15 May 2024; accepted on 18 May 2024
 
Abstract: 
In vitro propagation is a technique that has been used to produce large-scale, healthy, pathogen-free plants. However, the presence of endophytic microorganisms in explant tissues is one of the limiting factors for the in vitro establishment of any species. In this sense, we studied the effectiveness of PPM® biocide (Plant Preservative Mixture) and Chlorothalonil fungicide to control and/or reduce microbial and fungal contamination in mature nodal segments of different Persian lime clones under in vitro conditions. The results showed that explants cultured in MS medium (Murashige and Skoog 1962) supplemented with 0.2 mL L-1 of PPM biocide + 1.0 mL L-1 of Chlorothalonil were adequate to achieve a higher percentage of disinfection (44%) in mature nodal segments in the Persian lime clones. Furthermore, it was observed that PPM concentrations above 0.3 mL L-1 resulted in greater phytotoxicity in mature nodal segments at 60 days.
 
Keywords: 
Biocide; Citrus latifolia; Contamination; Disinfection; Endophytic microorganisms
 
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