Characterization of a drug resistant MCF-7 breast cancer cell line developed by repeated cycles of 5-Fluorouracil (5-FU) therapy

Erebi Patricia Tawari * and Melvin Peremobowei Ere

Department of Chemical Pathology, Faculty of Basic Clinical Sciences, College of Health Science, Niger Delta University, Bayelsa State, Nigeria.
 
Research Article
International Journal of Science and Research Archive, 2024, 13(02), 500–505.
Article DOI: 10.30574/ijsra.2024.13.2.2166
Publication history: 
Received on 29 September 2024; revised on 07 November 2024; accepted on 09 November 2024
 
Abstract: 
Breast cancer accounts for 14% of cancer fatalities and 23% of all cancer diagnoses, making it the most common cause of cancer death among women and one of the most frequently diagnosed cancers. This disease is a life-threatening public health concern because of its significant influence on the general population. Therefore, further molecular research is required to determine its prognosis and create tailored treatments. Since it closely resembles mammary epithelium, the human breast cancer cell line MCF-7, which expresses genes for oestrogen, progesterone, and glucocorticoid receptors, is the most appropriate in vitro model for cancer research. Resistant cell lines were created by repeatedly cultivating MCF-7 wild-type cells in media containing 5-fluorouracil, a first-line treatment for breast cancer. The resistant cell lines were found to be cross-resistance to other anticancer medications in addition to being resistant to 5-fluorouracil, according to the in vitro MTT cytotoxicity assay.
 
Keywords: 
MCF-7 cells; 5-Fluorouracil; Acquired resistance; Breast cancer cells
 
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